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在实验性缺血性中风中,母细胞稳定降低了血栓治疗药物后的出血形成和死亡率
Daniel Strbian1, Marja-Liisa Karjalainen-Lindsberg, Petri T Kovanen
1Department of Neurology, Helsinki University Central Hospital, Haartmaninkatu 8, 00290 Helsinki, Finland.
Circulation
|July 4, 2007
概括
大脑巨细胞 (MCs) 在中风的组织等离子体激活剂 (tPA) 治疗后导致出血和损伤. 稳定MCs减少了这些有害影响,改善了结果和生存率.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 血管生物学 血管生物学
背景情况:
- 组织等离子体激活剂 (tPA) 对于中风治疗至关重要,但可能导致出血并发症和再注射损伤.
- 大脑巨细胞 (MCs) 在脱粒时释放血管活性和蛋白质溶解物质,可能会加剧与中风相关的损伤.
- 以前的研究表明,MCs会增加缺血性和出血性脑 edem 和中性粒细胞的积累.
研究的目的:
- 为了研究巨细胞 (MCs) 在组织等离子体激活剂 (tPA) 介导的出血形成 (HF) 和再注射损伤中的作用.
- 评估巨细胞稳定在缓解tPA诱导并发症的治疗潜力.
主要方法:
- 在实验室中将MC暴露在tPA中,以评估脱粒化.
- 在老鼠体内焦点脑缺血/再输血模型.
- 药理学MC稳定使用cromoglycate.
- 对缺乏MC的老鼠的评估.
- 评估出血形成,大脑胀,中性粒细胞透,神经结果和死亡率.
主要成果:
- 在实验室中,tPA暴露诱导了显著的MC脱粒.
- 在老鼠中,tPA的发病后给药导致出血形成增加了70到100倍.
- 在24小时内,MC稳定用染色糖酸显著降低了tPA介导的HF,降低了96%.
- 缺乏MC的老鼠在24小时内显著减少了tPA介导的HF (89%).
- 无论是MC稳定还是缺乏,都减少了大脑胀,中性粒细胞透,改善了神经结果和生存率.
结论:
- 乳腺细胞在tPA注射后的出血形成和再注射损伤中发挥着重要作用.
- 巨细胞的药理稳定是一种有前途的治疗策略,可以提高血栓缓解疗法的安全性.
- 向巨细胞可以改善tPA治疗中风后的患者预后.
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