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Updated: Jul 6, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

可光激活和水溶性FRET染料,具有高脱的横截面

Genhua Zheng1, Yan-Ming Guo, Wen-Hong Li

  • 1Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.

Journal of the American Chemical Society
|August 10, 2007
PubMed
概括

No abstract available in PubMed .

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...