对于用于DNA度测定的化聚电解质的设计
Chunyan Chi1, Alexander Mikhailovsky, Guillermo C Bazan
1Department of Chemistry, Institute for Polymers and Organic Solids, University of California, Santa Barbara, California 93106, USA.
Journal of the American Chemical Society
|August 21, 2007
概括
新的阴离子联多电解质使敏感的双链DNA度确定成为可能. 这些聚合物在结合时改变颜色和排放,允许在广泛的DNA度范围内进行准确的量化.
科学领域:
- 聚合物化学 聚合物化学
- 生物物理化学 生物物理化学
- 分析化学 分析化学
背景情况:
- 在分子生物学和诊断中,开发用于确定双链DNA (dsDNA) 度的敏感和选择性方法至关重要.
- 现有的方法可能在灵敏度,动态范围上有局限性,或需要复杂的程序.
- 结合聚电解质提供独特的光学特性,可用于传感应用.
研究的目的:
- 设计,合成和表征用于dSDNA度确定的新型阴离子联聚电解质.
- 调查dSDNA结合时的光谱变化背后的光物理机制.
- 为了优化聚合物系统在dSDNA量化中具有广泛的动态范围.
主要方法:
- 合成和表征带离子结合的多电解质 (poly110和poly120) 与不同的2,1,3-二 (BT) 含量.
- 光谱分析 (光照发光) 用于研究多电解质和dSDNA之间的相互作用.
- 基于光谱变化 (辐射颜色变化,参数三角形) 的校准曲线的开发,以量化dsDNA度.
- 探索扩大可测量的dsDNA度范围的策略.
主要成果:
- 聚110和聚120在与dsDNA结合时呈现出明显的蓝色至绿色的发射颜色变化.
- 这些光谱变化归因于从烯-段到BT单元的Förster共振能量转移 (FRET) 的增强,BT量子产量的增加以及烯-排放的灭.
- 从光谱响应得出的校准曲线允许dSDNA的确定.
- 结合不同初始聚电解质度的数据,可以将可测量的dsDNA范围扩展到七个数量级.
结论:
- 阴离子联聚电解质poly110和poly120是有效的光探针,用于测量dSDNA度.
- 观察到的光谱变化为dsDNA存在提供了敏感和可量化的反应.
- 开发的系统显示出非常广泛的动态范围,使其适用于需要dDNA定量化的各种生物应用.
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