蓝光激活的氨酸激酶:细菌中的两组分传感器
Trevor E Swartz1, Tong-Seung Tseng, Marcus A Frederickson
1Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, CA, USA.
概括
科学家在像Brucella abortus这样的细菌中发现了光激活的histidine kinases. 这些胺结合蛋白质作为光受体,调节细菌的毒性,以应对光刺激.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 细菌生理学 细菌生理学
背景情况:
- 细菌的两组系统利用histidine激酶进行环境传感,调节基因表达,化学毒性,光毒性和毒性.
- 含有黄素的领域是已知的光感应模块在各种生物体,包括植物,藻类和真菌.
研究的目的:
- 为了研究光激活的histidine激酶在 prokaryotes 的存在和功能.
- 确定这些光受体在细菌毒性中的作用,特别是在Brucella abortus中.
主要方法:
- 在Brucella melitensis,Brucella abortus,Erythrobacter litoralis和Pseudomonas syringae中识别光激活的基酶.
- 弗拉结合和光化学的分析,包括乙烯-弗拉 adduct 形成.
- 在不同的光照条件下和突变菌株中评估B. abortus的巨细胞感染.
主要成果:
- 在研究的细菌物种中发现含有黄素染色体的光激活的丁激酶.
- 照相化学的证据与cysteinyl-flavin adduct形成一致.
- 在野生型菌株中对B. abortus巨菌感染的光刺激,在光受体突变体中减少.
结论:
- 含有黄素的胺基酶在细菌中作为光受体起作用.
- 这些光感应的histidine kinases调节细菌的毒性,正如B. abortus感染的光依赖调节所示.
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