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相关概念视频

Chemiosmosis01:32

Chemiosmosis

Oxidative phosphorylation is a highly efficient process that generates large amounts of adenosine triphosphate (ATP), the basic unit of energy that drives many cellular processes. Oxidative phosphorylation involves two processes— the electron transport chain and chemiosmosis.
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons reduce...
Preparation and Reactions of Sulfides02:26

Preparation and Reactions of Sulfides

Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
Microbial Mats01:25

Microbial Mats

Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...
Microbes and the Sulfur Cycle01:29

Microbes and the Sulfur Cycle

Sulfur is a vital element in Earth's biogeochemical systems. It transitions through various inorganic states, including sulfate (SO₄²⁻), elemental sulfur (S⁰), and sulfide (S²⁻). Abiotic and biological mechanisms across oxic and anoxic environments intricately mediate these transformations. Sulfate, the most oxidized form of sulfur, is predominantly stored in rocks, marine sediments, and oceanic waters, acting as a long-term reservoir in the global sulfur cycle.In oxic environments,...

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相关实验视频

Updated: Jun 30, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

在Dimethylsulfoxide中结和活性的Tetrahymena pyriformis.

S W Hwang, E E Davis, M T Alexander

    Science (New York, N.Y.)
    |April 3, 1964
    PubMed
    概括

    状原生动物,如Tetrahymena pyriformis,可以在极端寒冷中生存. 这项研究表明,使用两步冷过程和液储存成功保存,保持运动性和繁殖.

    科学领域:

    • 微生物学 微生物学
    • 低温生物学 低温生物学
    • 细胞生物学 细胞生物学

    背景情况:

    • 状原生动物是单细胞真核生物.
    • 在低温下保存微生物对于研究和生物技术至关重要.

    研究的目的:

    • 为了研究状原生动物的冷保存潜力.
    • 为了确定Tetrahymena pyriformis是否可以在深度冷后生存并保持功能.

    主要方法:

    • 泰特拉希梅纳pyriformis被悬浮在10%的二甲基硫氧化物 (冷保护剂) 中.
    • 采用了两步冷协议:-20°C,然后立即转移到 -196°C.
    • 样品被储存在液冰箱中.

    主要成果:

    • 泰特拉海梅纳形虫 (Tetrahymena pyriformis) 在两步冷过程中幸存下来.
    • 在解后,原生动物表现出恢复的移动性.
    • 在冷和储存后,原生动物的繁殖能力也恢复了.

    结论:

    • 使用二甲基硫氧化物的两步冷方法对冷保存Tetrahymena pyriformis有效.

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    Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
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    Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

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    Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe
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    相关实验视频

    Last Updated: Jun 30, 2026

    Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
    15:19

    Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

    Published on: October 15, 2015

    Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
    10:33

    Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

    Published on: October 15, 2018

    Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe
    08:07

    Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

    Published on: May 2, 2025

  • 这种技术可以在液中长期储存毛原生动物,同时保持生命力和生殖能力.