概括
这种细菌名为Agrobacterium tumefaciens.
科学领域:
- 植物分子生物学 植物分子生物学
- 微生物遗传学微生物遗传学
- 基因工程是一种基因工程.
背景情况:
- Agrobacterium tumefaciens通过其诱导瘤 (Ti) 等离子体在植物中引起皇冠胆病.
- Ti等离子体有两个关键区域:用于瘤形成的转移DNA (T-DNA) 和用于瘤发育的毒性区域.
研究的目的:
- 研究Ti等离子体作为植物遗传修饰的载体的潜力.
- 探索用于基因转移应用的Ti等离子体的修饰.
主要方法:
- 对Ti等离子体的诱导瘤和毒性区域的分析.
- 通过插入外来DNA来修改T-DNA区域.
- 从Ti等离子体中去除导致瘤的基因.
主要成果:
- 通过外来DNA插入,T-DNA区域转移到植物基因组的能力即使在通过外来DNA插入增加大小后也保持不变.
- 移除致瘤基因允许植物正常生长和分化.
- 修改后的Ti等离子体显示出作为基因转移载体的潜力.
结论:
- Ti等离子体可以被设计成一个功能载体,用于将外来基因转移到植物中.
- 这种方法促进了转基因植物的发展,而不会引起疾病症状.
相关概念视频
Transgenic Organisms
Overview
Non-nuclear Inheritance
Most DNA resides in the nucleus of a cell. However, some organelles in the cell cytoplasm—such as chloroplasts and mitochondria—also have their own DNA. These organelles replicate their DNA independently of the nuclear DNA of the cell in which they reside. Non-nuclear inheritance describes the inheritance of genes from structures other than the nucleus.
Plant Tissue Culture
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
Non-nuclear Inheritance
Most DNA resides in the nucleus of a cell. However, some organelles in the cell cytoplasm—such as chloroplasts and mitochondria—also have their own DNA. These organelles replicate their DNA independently of the nuclear DNA of the cell in which they reside. Non-nuclear inheritance describes the inheritance of genes from structures other than the nucleus.
Transgenic Organisms
Overview
Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...


