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相关概念视频

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

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相关实验视频

Updated: Jul 12, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

大豆种子释放的乳清素.

D W Fountain, D E Foard, W D Replogle

    Science (New York, N.Y.)
    |September 16, 1977
    PubMed
    概括

    在种子水分的最初8小时内,大豆莱克优先释放. 这种释放发生在不考虑种子活力或化物存在的情况下,表明这是一个被动的过程.

    科学领域:

    • 植物生物化学 植物生物化学
    • 种子生理学 种子生理学

    背景情况:

    • 大豆种子含有莱克,这些蛋白质具有碳水化合物结合特性.
    • 了解莱克释放对于种子加工和生物化学研究至关重要.

    研究的目的:

    • 为了研究在种子水化过程中大豆莱克的释放动力学.
    • 确定影响莱克释放的因素,如种子活力和化学抑制剂.

    主要方法:

    • 血凝试验被用来量化莱克活性.
    • 开发了一种过纸试验,以检测单个大豆种子释放的莱克.

    主要成果:

    • 大豆乳酸是一种在吸水后的前8小时内优先释放的产品.
    • 莱克的释放独立于种子的生存能力.
    • 释放过程对代谢抑制剂阿齐德不敏感.

    结论:

    • 大豆莱克在水合后很容易释放,主要是在早期阶段.
    • 释放机制似乎是一个被动的物理过程,而不是一个活跃的代谢过程.
    • 这一发现对大豆种子储存和莱克提取协议有影响.

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