相关实验视频
Updated: Jul 12, 2026

12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
一种激素的化学表征,该激素在复杂组织中促进细胞在g2中被捕
概括
研究人员确定了trigonelline (N-甲基尼古丁酸) 作为豆植物中的G2阶段细胞循环停止因子. 这标志着在复杂的植物和动物组织中首次化学识别了一种调节激素的细胞停止.
科学领域:
- 植物生理学 植物生理学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 细胞循环调节对生物体发育至关重要.
- 植物激素在生长和发育中起着至关重要的作用.
- 了解细胞循环控制机制对于植物科学至关重要.
研究的目的:
- 隔离和识别Pisum sativum cotyledons中的G2阶段增长阻断因子.
- 为了确定所识别的因子的化学特征.
- 研究其作为复杂组织中潜在激素的作用.
主要方法:
- 从Pisum sativum的G2因子的分离.
- 化学识别技术用于确定因子的结构.
- 细胞周期分析以确认G2阶段的生长停止.
主要成果:
- 一个G2因子被成功地从豆叶中分离出来.
- 隔离的因子被确定为三角蛋白 (N-甲基尼古丁酸).
- 特里戈内林 (Trigonelline) 证明了在细胞周期的G2阶段阻止细胞生长的能力.
结论:
- 特里戈内林是Pisum sativum中调节细胞循环进展的关键因素.
- 这是首次对一种激素进行化学鉴定,这种激素会导致复杂的植物和动物组织中的细胞循环停止.
- 特里戈内林代表了一类新的植物性细胞循环调节剂.
相关概念视频
Negative Regulator Molecules
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Molecular Factors Affecting Cell Division
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

