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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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相关实验视频

Updated: Jul 12, 2026

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
09:09

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Published on: March 5, 2021

打破衍射屏障:纳米尺度上的光学显微镜.

E Betzig, J K Trautman, T D Harris

    Science (New York, N.Y.)
    |March 22, 1991
    PubMed
    概括

    新的近场探测器显著提高了光学显微镜的分辨率和信号强度,使得分衍射成像成为可能. 这一突破将光学表征与纳米空间分辨率相结合,用于先进的材料分析.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 材料科学 材料科学 材料科学
    • 纳米技术纳米技术

    背景情况:

    • 近场扫描光学显微镜 (NSOM) 的目标是超越光的衍射极限.
    • 传统的NSOM面临的挑战是信号强度低,分辨率有限.
    • 用光学方法实现纳米空间分辨率对于高级表征至关重要.

    研究的目的:

    • 开发先进的近场探测器,以提高NSOM的性能.
    • 为了在NSOM成像中展示改进的分辨率和信号放大.
    • 在NSOM中研究图像对比的偏振依赖性.

    主要方法:

    • 开发具有子波长维度的新型近场探测器.
    • 使用接近 (lambda/50) 样品表面的探针.
    • 高空间分辨率的光学表征和成像.
    • 分析图像对比度变化与极化.

    主要成果:

    • 实现了大约12nm (lambda/43) 的空间分辨率.
    • 与以前的方法相比,观察到的信号放大10^4到10^6倍.
    • 展示了图像对比度的高极化依赖性.

    更多相关视频

    Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
    08:44

    Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

    Published on: August 22, 2017

    Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
    08:54

    Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

    Published on: June 5, 2019

    相关实验视频

    Last Updated: Jul 12, 2026

    Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
    09:09

    Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

    Published on: March 5, 2021

    Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
    08:44

    Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

    Published on: August 22, 2017

    Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
    08:54

    Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

    Published on: June 5, 2019

  • 通过新的探测器成功生成了高分辨率图像.

    • 结论:

    • 开发的近场探测器显著提升了NSOM的能力.
    • 提高分辨率和信号强度为广泛的NSOM应用铺平了道路.
    • 偏振依赖的对比为样本分析提供了新的途径.
    • NSOM准备将光学表征与纳米分辨率集成在一起.