概括
循环赫西米德脉冲通过抑制蛋白质合成来改变神经的昼夜时钟. 缺乏这种抑制的耐药突变体没有表现出时钟相位转移,证实了该机制.
科学领域:
- * * 时间生物学
- * 分子遗传学 分子遗传学
- * 菌群学 * 菌群学
背景情况:
- * 循环时钟调节每天的生物节奏.
- * 蛋白质合成对细胞功能和节律性至关重要.
- * 循环赫西米德是已知的蛋白质合成抑制剂.
研究的目的:
- * 调查蛋白质合成在昼夜钟调节中的作用.
- * 为了确定循环赫西米德诱导的神经的相位转移是否通过蛋白质合成抑制进行介导.
主要方法:
- *向神经虫进行小,间歇性剂量 (脉冲) 循环赫西米德的施用.
- *观察和测量昼夜时钟的相位变化.
- * 使用具有改变80S核糖体的抗循环赫西米德Neurospora突变体.
主要成果:
- * 循环赫西米德脉冲诱导了神经的昼夜时钟的显著相位变化.
- * 耐循环赫西米德突变体在药物暴露时没有表现出昼夜时钟相位变化.
- *突变体的抗性归因于它们的80S核糖体.
结论:
- * 抑制蛋白质合成是循环赫西米德影响神经的昼夜时钟的主要机制.
- * 昼夜时钟的阶段对蛋白质合成中断非常敏感.
- *80S核糖体参与调解环胺对昼夜节律的影响.
相关概念视频
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Biological Clocks and Seasonal Responses
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
Gene Regulation During Sporulation
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
From DNA to Protein
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...


