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一个子突变的阿拉比多普西斯由T-DNA插入突变产生突变

K A Feldmann, M D Marks, M L Christianson

Science (New York, N.Y.)

|March 10, 1989

概括

研究人员描述了Arabidopsis thaliana中一个矮突变的特征,确定了一个单一的衰退核突变. 这种突变影响了植物的高度和共分离,具有卡纳米辛耐药性和T-DNA插入,有助于发现复杂特征的基因.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农业学是一种农业学.

背景情况:

许多控制复杂农学特征的植物基因仍然没有表征.
目前用于隔离这些基因的分子方法受到基因产品知识缺乏的限制.

研究的目的:

为了描述由Agrobacterium介导的转变导致的Arabidopsis thaliana的表型变化.
识别和描述影响植物发育和农学特征的特定突变.

主要方法:

阿拉比多普西斯塔利亚纳的种子被Agrobacterium感染,产生了转变的线条.
对136个转变系进行了调查,以确定稳定的表型变化.
进行分离分析以确定改变表型的遗传模式,卡纳米辛耐药性和T-DNA插入.

主要成果:

转变的线条表现出高度,花朵结构,三合体,游戏生殖,胚胎生殖和幼苗发育的稳定表型变化.
一个特定的矮子突变体的特征,显示遗传作为一个单一的衰退核突变.
这种矮体现型与卡纳米辛耐药性和T-DNA插入物共同分离.

结论:

农业细菌介导的转化是一种有效的工具,用于产生和表征影响复杂植物特征的突变.
鉴定到的矮突变物为了解植物身高的遗传控制提供了宝贵的资源.
这种方法通过共同分离分析来促进控制重要的农学特征的基因的隔离.

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