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相关概念视频

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent – the...

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相关实验视频

Updated: Jul 11, 2026

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
10:48

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

Published on: December 17, 2017

材料:在微重力下,蛋白质单晶生长.

W Littke, C John

    Science (New York, N.Y.)
    |July 13, 1984
    PubMed
    概括

    制造用于X射线结晶学的大型蛋白质晶体具有挑战性. 微重力条件显著增强了β-galactosidase和lyszyme的晶体大小,产生了比地球上生长的晶体大27和1000倍的晶体.

    科学领域:

    • 生物物理学的生物物理.
    • 晶体学 晶体学是指结晶学.
    • 材料科学 材料科学 材料科学

    背景情况:

    • 蛋白质单晶制备对于X射线结构的确定至关重要.
    • 晶体的生长通常受到陆地重力诱导的对流和沉积的限制.
    • 开发用于更大,更高质量的晶体的方法对于结构生物学至关重要.

    研究的目的:

    • 为了研究微重力对蛋白质单晶生长的影响.
    • 为了比较在微重力下获得的晶体大小与地面重力条件.
    • 评估微重力的潜力,以改善蛋白质结晶学工作流程.

    主要方法:

    • 利用微重力条件生长两个模型蛋白质的单晶:β-galactosidase和lyszyme.
    • 在结晶过程中采用了脱盐方法.
    • 在晶体形成过程中保持没有对流的溶液.
    • 结晶体量与在地球引力下同时生长的结晶体量进行比较.

    主要成果:

    • 在微重力条件下成功地产生了更大的β-银酸酶和酶单晶.
    • 微重力培养的β-银酸酶晶体体的体积是地面对照的27倍.
    • 微重力培养的酶晶体的体积是地面对照的1000倍.

    更多相关视频

    Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
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    Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
    09:28

    Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

    Published on: August 25, 2022

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    In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
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    Published on: December 17, 2017

    Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
    09:15

    Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

    Published on: August 14, 2018

    Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
    09:28

    Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

    Published on: August 25, 2022

  • 在微重力下的无对流条件促进了增强的晶体生长.

    • 结论:

    • 微重力显著增加了蛋白质单晶体的大小.
    • 在微重力下没有对流是改善晶体生长的关键因素.
    • 微重力提供了一种有希望的方法来克服结构研究中蛋白质晶体制备的局限性.