相关实验视频

Updated: Jul 11, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

在近场光学显微镜中单个分子光寿命的变化

W P Ambrose, P M Goodwin, R A Keller

    Science (New York, N.Y.)
    |July 15, 1994
    PubMed
    概括

    在二氧化上测量了罗达6G单分子光寿命. 近场扫描光学显微镜显示,接近探头会改变光衰变,这表明表面附近的火效应.

    更多相关视频

    Fluorescence Lifetime Macro Imager for Biomedical Applications06:01

    Fluorescence Lifetime Macro Imager for Biomedical Applications

    Published on: April 7, 2023

    Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules10:23

    Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

    Published on: April 25, 2025

    相关概念视频

    Protein Dynamics in Living Cells01:19

    Protein Dynamics in Living Cells

    Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
    Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
    Super-resolution Fluorescence Microscopy01:37

    Super-resolution Fluorescence Microscopy

    Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.