对于缺血性心脏病的胚胎干细胞衍生的内皮细胞的分化,生存和功能
Zongjin Li1, Jenny C Wu, Ahmad Y Sheikh
1Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Circulation
|September 14, 2007
概括
胚胎干细胞衍生的内皮细胞 (ESC-ECs) 在治疗心脏病发作方面表现有前途. 这些细胞在小鼠中存活了8周,通过促进新血管生长,改善了心脏功能.
科学领域:
- 心血管研究研究心血管研究
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 胚胎干细胞具有自我更新和多能性.
- 对ES细胞衍生的内皮细胞 (ESC-ECs) 的表征对于治疗应用至关重要.
- 研究ESC-EC生存率和活体疗效对于心脏修复至关重要.
研究的目的:
- 用分子成像来描述ESC-EC并评估它们的生存率.
- 确定ESC-ECs在缺血性损伤后恢复心脏功能方面的治疗疗效.
- 评估ESC-ECs作为心肌缺血细胞治疗的潜力.
主要方法:
- 鼠ES细胞分化为ESC-ECs,通过FACS分析确定 (Flk-1,VE-cadherin).
- 通过使用生物发光 (Fluc) 和光 (mRFP) 报告器,ESC-ECs被设计为体内追踪.
- 将ESC-EC移植给心肌梗塞的小鼠,并通过生物发光成像,心声学和组织学来评估结果.
主要成果:
- 分化ESC-EC表达了内皮标记物,并形成了血管类结构.
- 生物发光成像证实ESC-EC存活在移植后大约8周.
- 在心脏梗塞中,ESC-EC移植显著改善了心脏功能,并增加了毛细血管密度.
结论:
- 这项研究证明了在心脏中跟踪移植的ESC-EC的可行性.
- ESC-EC显示出诱导血管生成和治疗心肌缺血的治疗潜力.
- 对于未来的心脏再生疗法来说,ESC-EC 是一个有前途的细胞来源.
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