在DNA细胞因子-细胞因子不匹配中插入一个庞大的复合物:一个NMR溶液研究研究
Christine Cordier1, Valérie C Pierre, Jacqueline K Barton
1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Journal of the American Chemical Society
|September 20, 2007
概括
重的Rh(bpy) 2chrysi3+金属复合物通过小道插入DNA不匹配,将不匹配的基射入主要道. 这种结合极小地扭曲了溶液中的DNA结构,NMR证实了这一点.
科学领域:
- 生物有机化学 生物有机化学
- 结构生物学 结构生物学
- 分子生物物理学 分子生物物理学
背景情况:
- 大量的金属复合体可以针对DNA不匹配.
- 了解结合模式对于开发DNA向剂至关重要.
研究的目的:
- 描述Rh(bpy) 2chrysi3+在DNA中具有CC不匹配的溶液状态结合模式.
- 为相互作用机制提供结构性证据.
主要方法:
- 核磁共振 (NMR) 谱学是指核磁共振的光谱学.
- 2D NOESY 和 COSY 实验中的二维 NOESY 和 COSY.
- 31P 核磁共振是指 31P 的核磁共振.
主要成果:
- Rh(bpy) 2chrysi3+ 插入到CC不匹配部位的DNA小槽中.
- 不匹配的细胞因子被射出到主要槽中.
- DNA局部构造和糖 (C2'-endo) 在很大程度上保持不变.
- 酸盐骨干从BI过渡到BI构成,促进了复杂的插入.
结论:
- 核磁共振 (NMR) 证实了在DNA不匹配处的大型金属复合物的深小槽插入.
- 结合机制涉及最小的DNA扭曲和特定的脊柱形状变化.
- 溶液结构数据支持这种DNA结合复合体的晶体学发现.
更多相关视频
11:37Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
Published on: July 28, 2017
09:04Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
Published on: July 26, 2018
相关概念视频
01:20Mismatch Repair
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
01:36Mismatch Repair
Overview
01:13¹H NMR: Complex Splitting
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.