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Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
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需要氨酸来释放Xenopus蛋提取物从介质M阶段的介质M阶段
1Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK.
Nature
|September 21, 2007
概括
受精会触发卵子中的 (Ca2+) 激增,激活氨酸酸酶. 这种酶对于释放细胞循环从停滞中至关重要,使其能够通过半变和介质阶段的进展.
科学领域:
- 细胞生物学 细胞生物学
- 发育生物学 发展生物学
- 生物化学 生物化学
背景情况:
- 受精触发了动物卵中的细胞质Ca2+的暂时增加,使它们从介质性停止中释放出来.
- Ca2+激活了Ca2+/calmodulin激活的激酶,使细胞静止因子失活,并允许激活促进酶因子.
研究的目的:
- 为了研究激活蛋白质酸酶氨酸在受精后的卵细胞循环重新进入中的作用.
- 为了识别与介质退出有关的酸酶的基质和调节.
主要方法:
- 利用青蛋提取物研究Ca2+诱导的细胞循环事件.
- 服用氨酸抑制剂 (例如,环素A) 来评估它们对蛋白质脱和细胞循环进展的影响.
- 监测了蛋白质无处不在,蛋白水平和核包膜改造.
主要成果:
- 氨酸在蛋提取物中被Ca2+暂时激活.
- 氨酸抑制延迟了环林的破坏,M相蛋白脱,以及核包膜的重新形成.
- 酸酶活动的第二波,与氨酸不同,准线性酸蛋白,对于及时进入相间阶段至关重要.
结论:
- 氨酸在打破莫斯-MAP激酶诱导的介质性停滞方面发挥着关键作用.
- Fizzy/Cdc20被确定为介质退出过程中氨酸的关键基质.
- 一个受调节的酸酶级联,涉及氨酸和第二个酸酶,对于成功从M阶段向中间阶段的进展至关重要.
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