甲基酸盐是植物系统性获得抵抗的关键移动信号
Sang-Wook Park1, Evans Kaimoyo, Dhirendra Kumar
1Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA.
概括
甲基酸盐 (MeSA) 被认为是引发植物系统性获得性抵抗 (SAR) 的移动信号分子. 这一发现澄清了植物如何系统地增强对病原体的防御.
科学领域:
- 植物病理学 植物病理学
- 分子植物微生物相互作用.
- 植物的信号传输.
背景情况:
- 系统性获得耐药性 (SAR) 是植物对病原体的重要防御机制.
- 负责SAR的移动信号分子仍然未被确定.
- 了解SAR信号对于开发可持续的作物保护策略至关重要.
研究的目的:
- 为了识别移动信号分子,在植物中调解系统性获得抵抗 (SAR).
- 阐明酸结合蛋白2 (SABP2) 在SAR感知中的作用.
- 调查甲基酸盐 (MeSA) 在SAR诱导中的参与.
主要方法:
- 使用不同遗传背景的马赛克烟草植物进行移植实验.
- 对SABP2在免疫刺激反应中的MeSA酶活性进行分析.
- 在初级受感染的叶子中,SA甲基转移酶的基因沉默.
- 用MeSA进行治疗,以评估系统组织中的SAR诱导.
主要成果:
- SABP2的MeSA酶活性对于系统组织中SAR信号感知至关重要.
- 突变SABP2具有不受管制的活动,影响了SAR和降低了MeSA水平.
- 沉默SA甲基转移酶阻断了SAR,而MeSA治疗则系统性诱导了SAR.
- 检测到MeSA是在主要感染的叶子的叶膜液中检测到的.
结论:
- 甲基酸盐 (MeSA) 被证实是诱导烟草系统性获得性耐药性 (SAR) 的移动信号分子.
- 在系统组织中,SABP2在感知MeSA信号方面发挥着关键作用.
- MeSA/SA代谢途径是SAR信号转导的组成部分.
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