在Agrobacterium转换中的特洛伊木马策略:滥用MAPK防御信号
Armin Djamei1, Andrea Pitzschke, Hirofumi Nakagami
1Department of Plant Molecular Biology, Max F. Perutz Laboratories, University of Vienna, Dr.-Bohr-Gasse 9, 1030 Vienna, Austria.
Science (New York, N.Y.)
|October 20, 2007
概括
菌 (Agrobacterium tumefaciens) 劫持了植物的防御路径. 毒性蛋白VirE2使用MPK3酶准的转录因子VIP1,将T-DNA输送到核中.
科学领域:
- 植物分子生物学 植物分子生物学
- 微生物的病原发生.
- 蜂信号传输是如何进行的
背景情况:
- 核进口的T-DNA对于Agrobacterium介导的植物转化至关重要.
- 病毒性蛋白 VirE2 与宿主因子 VIP1 相互作用,促进 T-DNA 核导入.
- VIP1在植物细胞中的确切作用以前是未知的.
研究的目的:
- 为了阐明VIP1在植物细胞中的功能.
- 调查Agrobacterium利用VIP1进行T-DNA核导入的机制.
- 了解植物防护信号与Agrobacterium转化之间的相互作用.
主要方法:
- 确定了VIP1作为转录因子和MPK3中位基因激活蛋白激酶 (MAPK) 的直接标.
- 证明MPK3依赖酸化导致VIP1从细胞质转移到细胞核.
- 表明VIP1调节了与PR1致病相关的基因的表达.
主要成果:
- VIP1是一种由植物MAPK MPK3调节的转录因子.
- 由MPK3对VIP1的酸化对于Agrobacterium T-DNA的核进口至关重要.
- 亚格罗细菌利用MAPK激活的VIP1通路作为T-DNA传递的特洛伊木马.
结论:
- Agrobacterium tumefaciens劫持了植物的防御信号通路,涉及VIP1和MPK3,以进行高效的T-DNA核导入.
- VIP1作为一个关键的宿主因素,将植物防御反应与微生物转化联系起来.
- 了解这种机制,可以了解植物微生物相互作用,以及改善作物的潜在策略.
更多相关视频
10:28Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
11:16A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
相关概念视频
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
00:53Transgenic Organisms
Overview
01:07MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...