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相关概念视频

ATP and Macromolecule Synthesis01:28

ATP and Macromolecule Synthesis

Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

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Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

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用于聚胺合成的自组装模板.

Maxim G Ryadnov1, Derek N Woolfson

  • 1School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK. Max.Ryadnov@bristol.ac.uk

Journal of the American Chemical Society
|October 24, 2007
PubMed
概括
此摘要是机器生成的。

这项研究将化学结合与自组装纤维相结合,以创建极长的多链. 这种新的方法克服了用特定序列合成长蛋白质分子的挑战.

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Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets
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科学领域:

  • 生物化学 生物化学
  • 材料科学 材料科学 材料科学
  • 合成生物学 合成生物学

背景情况:

  • 具有精确序列的长多链 (>50氨基酸) 的化学合成是一个重大挑战.
  • 原生化学结合 (NCL) 是一种连接短的方法,但它对于非常长的链条有局限性.
  • 体自我组装提供了一种组织分子以进行后续反应的方法.

研究的目的:

  • 开发一种用于合成具有规定的序列的极长多链的方法.
  • 将化学结合与自组合相结合,以克服当前合成技术的局限性.
  • 创建长度超过10微米和质量超过3MDa的多链.

主要方法:

  • 利用自组装纤维 (SAF) 系统来组织成千上万的.
  • 在SAF中引入具有C-终端二子部分的,用于结合.
  • 在SAFs中的N-和C-终端处促进化学结合,绕过了对催化氨酸的需求.
  • 在结合后拆解非共价SAF组件以产生延长的多链.

主要成果:

  • 成功地产生了极长的多链 (>或=10微米) 与规定的,重复的序列.
  • 合成的分子估计质量大于或等于3 MDa.
  • 通过生物物理,水力动力学和显微镜测量来表征长长的多链.
  • 在自组装的结构中证明了高效的化学结合.

结论:

  • 化学结合和自组合的结合是合成超长多链的强大策略.
  • 这种方法克服了合成的关键挑战,使得能够创建大,序列定义的宏分子.
  • 开发的技术在创造新的生物材料和基于蛋白质的结构方面具有潜在的应用.