骨髓中自我更新的骨质原生体可以组织出一个造血微环境
Benedetto Sacchetti1, Alessia Funari, Stefano Michienzi
1Deparment of Experimental Medicine, La Sapienza University, 00161 Rome, Italy.
Cell
|October 25, 2007
概括
研究人员在骨髓层中确定了MCAM/CD146+亚内皮细胞,这些细胞在骨髓层中建立了造血微环境 (HME). 这些细胞对于HME的形成和骨祖先在新骨部位的移植至关重要.
科学领域:
- 血液学 血液学 血液学
- 干细胞生物学 干细胞生物学
- 血管生物学 血管生物学
背景情况:
- 构成人类骨髓血液生成微环境 (HME) 的细胞组件和骨髓层内的骨祖先仍然不太清楚.
- 识别这些细胞对于理解血液形成和骨再生至关重要.
研究的目的:
- 为了确定负责在人类骨髓中确定HME的特定细胞类型.
- 为了研究这些细胞在骨架原始细胞移植中的作用及其与血管系统的相互作用.
主要方法:
- 从人类骨髓中移植到异型位点的MCAM/CD146表达细胞.
- 在体内观察HME和微型骨器官的形成.
- 细胞间干细胞和发育中的鼻状体之间的细胞相互作用的分析.
- 评估由子内皮层层细胞产生的 ангиопоэтин-1.
主要成果:
- 来自人类骨髓层的MCAM/CD146表达子内皮细胞在移植后可以将HME转移到异型位点.
- 这些细胞建立了功能性微型骨器官,其中含有类似的子内皮细胞.
- 亚内皮层层细胞移植涉及与正在发展的鼻状细胞的动态相互作用.
- 这些层细胞是安吉奥波伊-1的重要生产者,这是HSC位和血管重塑的关键调节者.
结论:
- MCAM/CD146+子内皮细胞是造血微环境的关键编排者.
- 这些细胞在骨祖先的建立和骨髓新生代中发挥着至关重要的作用.
- 这些发现阐明了HME形成,骨原始体在 sinusids中的定位和血管生成之间的功能联系.
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