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基于的β-hairpin水凝具有固有的抗菌活性

Daphne A Salick¹, Juliana K Kretsinger, Darrin J Pochan

¹Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA.

Journal of the American Chemical Society

|November 8, 2007

概括

MAX1水凝支架表现出针对常见医院细菌的广泛抗菌活性. 这种自我组装的材料具有固有的抗微生物特性,对于组织再生应用中的感染控制至关重要.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
微生物学微生物学

背景情况:

生物材料植入带有很大的感染风险.
开发具有固有的抗菌性质的材料对于预防植入物相关感染至关重要.

研究的目的:

设计和评估一种具有固有的抗菌活性,用于组织再生的自组合基架 (MAX1).
研究抗菌作用的机制,并评估材料对哺乳动物细胞的安全性.

主要方法:

MAX1水凝被挑战了广泛的细菌度 (2 x 10^3到2 x 10^9 CFU/dm^2).
使用活死试验和共聚焦显微镜对阳性和阴性细菌进行了抗菌活性测试.
通过监测β-galactosidase释放来评估细菌膜的破坏.
用哺乳动物细胞的共同培养实验和用人类红细胞的血液溶解试验来评估细胞毒性.

主要成果:

MAX1水凝表面表现出广泛的抗菌活性,对抗患病在医院获得的细菌,包括白杆菌,黄金白杆菌,白杆菌,Klebsiella pneumoniae和大肠杆菌.
活死体测定证实了与表面接触时的细菌杀死,有证据表明内外膜被破坏.
共同培养实验表明选择性毒性对细菌超过哺乳动物细胞.
马克斯1水凝是非溶血性的,保留了人体红细胞的形态.

结论:

自组合MAX1形成具有强大,广泛的抗菌活性的水凝支架.
抗菌机制涉及细菌接触时直接破坏膜.
MAX1水凝具有选择性毒性,对细菌有害,但对哺乳动物细胞和红细胞安全.
这些特性使MAX1水凝对组织再生非常有希望,即使在非无菌的环境中.

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