在使用进化特征的12个Drosophila基因组中发现了功能元素
Alexander Stark1, Michael F Lin, Pouya Kheradpour
1The Broad Institute, Massachusetts Institute of Technology and Harvard University, Cambridge, Massachusetts 02140, USA.
Nature
|November 13, 2007
概括
对12个多虫物种的比较基因组学揭示了发现功能元素的进化特征. 这种方法可以识别新的基因,调节动机和microRNA基因,提高基因组注释的准确性.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 生物信息学是一种生物信息学.
背景情况:
- 对比基因组学对于理解基因组功能至关重要.
- 分析多种相关物种有助于识别保存和新型基因组元素.
研究的目的:
- 通过比较基因组学,在Drosophila中重新发现功能性基因组元素.
- 描述不同功能元素的进化特征.
- 评估物种数量和分歧对发现能力的影响.
主要方法:
- 12种多虫物种的全基因组测序.
- 进行比较基因组学分析以识别进化特征.
- 计算预测蛋白质编码基因,非编码RNA和调节动机.
主要成果:
- 识别了新的蛋白质编码基因,外子和不寻常的基因结构,包括停止-codon读透.
- 发现了新的microRNA基因,并为其处理和功能提供了证据.
- 高可靠性预测了许多转录前和转录后的调节动机.
- 展示了发现能力如何随着物种分歧和数量的变化而变化.
结论:
- 比较基因组学为全面的基因组注释提供了一个强大的框架.
- 进化签名是各种功能元素准确识别的关键.
- 建立了优化比较基因组研究的指导方针.
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