在没有聚合物溶解的DNA杂交体中发生了尖的融化过渡:证明了邻近双重合作的证明
Julianne M Gibbs-Davis1, George C Schatz, SonBinh T Nguyen
1Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
Journal of the American Chemical Society
|November 22, 2007
概括
合物聚合物-DNA杂交物显示出用于DNA检测的尖融化过渡. 邻近双层互动对这种合作和总体稳定有着显著的贡献.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 分子生物学分子生物学
背景情况:
- 合聚合物-DNA混合物,就像DNA修饰的金纳米颗粒一样,表现出尖的融化过渡.
- 这些转换对于开发高度选择性的DNA检测系统至关重要.
- 现有的理论将融归因于聚合相变或邻近双重相互作用.
研究的目的:
- 为了区分聚合物溶解和邻近双重相互作用对急剧融化过渡的贡献.
- 设计和分析一个聚合物-DNA混合聚合物系统,包括连接和不连接的双重复合体.
主要方法:
- 聚合物-DNA混合聚合物系统的制造.
- 混合系统的热分析 (化过渡).
- 使用简单的热力学模型进行定量评估.
主要成果:
- 聚合物连接的和部分不连接的DNA复合体都显示出的融化过渡.
- 部分不受约束的DNA复杂体表明,邻近的双重体相互作用会诱导合作性.
- 一个热力学模型量化评估了邻近双重效应和聚合性质的贡献.
结论:
- 邻近双重相互作用是聚合物-DNA聚合物的合作融化的关键因素.
- 这些聚合物的增强稳定来自邻近双重相互作用和多价值/聚合物特性.
- 这项研究为理解和优化基于聚合物-DNA混合体的DNA检测系统提供了一个框架.
相关概念视频
02:27DNA Base Pairing
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
02:05DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
02:27DNA Base Pairing
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
01:59Lagging Strand Synthesis
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
02:13Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
01:03Single-Strand DNA Binding Proteins
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...