抑制性细胞因子IL-35有助于调节T细胞功能
Lauren W Collison1, Creg J Workman, Timothy T Kuo
1Department of Immunology, St Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Nature
|November 23, 2007
概括
调节性T细胞利用一种新型的细胞因子,IN-35 (IL-35) 抑制免疫反应. 这一发现为免疫调节和自身免疫性疾病的潜在治疗点提供了新的见解.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 调节性T (Treg) 细胞对于免疫平衡和预防自身免疫是至关重要的.
- 然而,Treg细胞也抑制有益的免疫反应,介导其抑制功能的分子在很大程度上是未知的.
- 了解Treg细胞抑制机制对于免疫治疗的发展至关重要.
研究的目的:
- 确定介导调节性T细胞抑制活性的新型分子.
- 描述这些分子在免疫抑制中的功能和调节.
- 探索这些发现的潜在治疗影响.
主要方法:
- 对Treg和效应T细胞的基因表达分析.
- 共同培养实验以评估细胞因子的产生.
- 在小鼠身上进行了基因淘汰研究 (Ebi3-/- 和 Il12a-/-).
- 在体外和体外功能测定用于调节活性.
主要成果:
- 埃普斯坦-巴尔病毒诱导的基因3 (Ebi3) 和介质素-12α (Il12a) 在小鼠Treg细胞中高度表达,但不是效应T细胞.
- 一种Ebi3-IL-12alpha异构体,被指定为互白素-35 (IL-35),由Treg细胞分泌.
- 在体外和体内,IL-35是最大Treg细胞抑制活性所需的,包括控制炎症性肠病.
- 艾比3的表达受转录因子Foxp3的调节,证实了Treg细胞的特异性生产.
结论:
- 介素-35 (IL-35) 是一种新型的抑制性细胞因子,专门由调节性T细胞产生.
- IL-35在调解Treg细胞介导的免疫抑制中发挥着关键作用.
- 在各种疾病中,IL-35代表了调节免疫反应的潜在治疗标.
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