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Updated: Jul 9, 2026

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
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Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells

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单标签的激酶和酸酶测定用于使用纳秒时间解析的光检测的氨酸酸化

Harekrushna Sahoo1, Andreas Hennig, Mara Florea

  • 1School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, D-28759 Bremen, Germany.

Journal of the American Chemical Society
|November 30, 2007
PubMed
概括

使用2,3-diazabicyclo[2.2.2]oct-2-ene (Dbo) 光灭的新型试验测量了氨酸激酶和酸酶活性. 这种方法具有高灵敏度和广泛适用于酶活性监测和抑制剂查.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...