核糖体蛋白S3:NF-kappaB复合体中的KH域子单元,它调解了选择性基因调节
Fengyi Wan1, D Eric Anderson, Robert A Barnitz
1Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA.
Cell
|November 30, 2007
概括
研究人员发现核糖体蛋白S3 (RPS3) 是NF-kappaB蛋白质复合体的一个新子单元. RPS3增强了DNA结合,对于NF-kappaBB来说至关重要.
科学领域:
- 分子生物学分子生物学
- 细胞信号传输 细胞信号传输
- 蛋白质组学是指蛋白质组学.
背景情况:
- 核因子-kappaB (NF-kappaB) 是一个重要的转录因子复合体.
- NF-kappaB调节基因表达,以响应各种细胞信号.
- 了解NF-kappaB的选择性基因向对于细胞激活至关重要.
研究的目的:
- 为了识别NF-kappaB p65复合物的新型子单元.
- 阐明在NF-kappaB函数中识别的子单元的作用.
- 了解NF-kappaB优先基因招募的机制.
主要方法:
- 采用了与质谱学 (TAP-MS) 结合的并列亲和力净化.
- 在蛋白质组查中,NF-kappaB p65被用作诱.
- 进行了 Knockdown 实验,以评估已识别的蛋白质的功能.
主要成果:
- 核糖体蛋白S3 (RPS3) 被确定为NF-kappaB p65复合物的新型非Rel子单元.
- RPS3协同增强NF-kappaB的DNA结合活性.
- RPS3敲除特别损害了NF-kappaB介导的目标基因转录,而不影响核穿或全球翻译.
结论:
- RPS3是NF-kappaB的一个基本的,以前未被识别的子单元.
- 在快速细胞激活过程中,RPS3在调节特定基因方面发挥着关键作用.
- 这些发现为NF-kappaB.B.对选择性基因表达控制提供了洞察力.
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