强力诱导的细胞质氨酸的双向步进
Arne Gennerich1, Andrew P Carter, Samara L Reck-Peterson
1The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158-2200, USA.
Cell
|November 30, 2007
概括
酵母细胞质的dynein以8纳米的步骤移动,但可以逆行反抗力. 值得注意的是,dynein表现出核酸独立的运动,揭示了对运动协调至关重要的强力驱动的脱离-附着循环.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 分子电机分子电机
背景情况:
- 细胞质体dynein是一个重要的减值终端导向的微管子电机.
- 它的运动和力量生成的精确机制尚未完全理解.
研究的目的:
- 用光学子来研究酵母细胞质二烯的依赖力量的步进行为.
- 为了阐明在过程性运动过程中dynein的运动领域的协调.
主要方法:
- 使用光学子,对酵母细胞质染色素施加可控的力.
- 分析了运动蛋白的步进行为和取决于力量的运动性.
主要成果:
- 迪尼因主要以8纳米的步骤移动,其变化范围从4-24纳米.
- 反对力量增加后退步骤,在负载>7 pN时发生逆转.
- 在负载下证明了核酸独立的过程步进,表明力驱动的循环.
结论:
- 仅仅是力量可以驱动dynein的脱离-附着循环.
- 这些发现表明了dynein中运动域协调的模型.
- 提供了关于细胞过程中基于dynein的运动性的新见解.
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