在本地表皮表皮解体体中,卡德林的分子结构
Ashraf Al-Amoudi1, Daniel Castaño Díez, Matthew J Betts
1European Molecular Biology Laboratory, Meyerhofstr. 1, 69117 Heidelberg, Germany.
Nature
|December 8, 2007
概括
研究人员使用冷电子断层扫描在人体皮肤中可视化了desmosomal cadherins. 这揭示了详细的分子结构,解释了干德林分子如何相互作用以调节细胞粘附.
科学领域:
- 细胞生物学 细胞生物学
- 结构生物学是结构生物学.
- 生物物理学的生物物理.
背景情况:
- 德斯莫索姆是皮肤和心脏等组织中关键的细胞间结点.
- 不过,我们还没有完全理解索体中介干素粘附的精确分子机制.
研究的目的:
- 为了阐明人类表皮中的desmosomal cadherins的3D分子结构.
- 为了揭示细胞粘附中的卡德林相互作用的结构基础.
主要方法:
- 人体表皮的玻璃部分的冷电子断层扫描 (cryo-ET).
- 3D图像处理和子断层图像的平均值.
- 原子模型适合C-cadherin结构.
主要成果:
- 可视化了沿着desmosome中线的正规数组密度,类似于C-cadherin结构.
- 揭示了跨 (细胞间) 和cis (细胞内) 阴素相互作用的周期安排.
- 开发了一种3D模型,介绍了德斯莫索体内的德林组织.
结论:
- 这项研究提供了前所未有的3D分子细节的desmosomal cadherin组织.
- 拟议的模型解释了现有的二维数据,并提供了对基于cadherin的粘附机制的见解.
- 这项工作推动了我们对表皮组织中细胞-细胞粘附的理解.
相关概念视频
The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
Integrins
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Desmosomes
The term desmosome derives from the Greek words "desmo" and "soma" meaning "adhesion bodies." This structure was first observed during the late 1800s and described as small, dense nodules in the epidermis. Desmosomes are button-like structures that help form an interlinked network of intermediate filaments across the cells. These junctions are essential to hold cells together under mechanical stress and to maintain tissue integrity. Desmosomes are multi-protein complexes comprising desmosomal...
Reticular Dermis
The papillary and reticular dermis are the two layers of the dermis. They are made of connective tissue with fibers of collagen extending from one to the other, making the border between the two somewhat indistinct. The dermal papillae extending into the epidermis belong to the papillary layer, whereas the dense collagen fiber bundles below belong to the reticular layer.
Reticular Layer
Underlying the papillary layer is the much thicker reticular layer, composed of dense, irregular connective...
Reticular Layer
Underlying the papillary layer is the much thicker reticular layer, composed of dense, irregular connective...
Fibrous Proteins
Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...


