通过COMPASS介导的H2B单双化和H3甲基化之间的基因组交叉
Jung-Shin Lee1, Abhijit Shukla, Jessica Schneider
1Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA.
Cell
|December 18, 2007
概括
素H2B单双化控制了通过Cps35.5的COMPASS复合结合. 这种基本的子单元将H2B修饰与H3K4和H3K79甲基化联系起来,揭示了一个关键的基因素交叉交叉机制.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 康帕斯是一种酵母组织基因组甲基转移酶复合体,负责H3K4甲基化.
- 素H2B单双化对于H3K4和H3K79甲基化至关重要,但其机制尚不清楚.
- Cps35是COMPASS综合体中唯一的基本子单位.
研究的目的:
- 为了阐明将基因素H2B单双化与COMPASS介导的H3甲基化联系起来的分子机制.
- 为了研究Cps35在调解基因组交叉通话中的作用.
主要方法:
- 生物化学净化 COMPASS 综合体的生物化学净化.
- 对野生类型和突变酵母菌株 (例如Deltarad6) 的COMPASS活性进行分析.
- 对Cps35.5的染色体关联研究.
主要成果:
- 素H2B单双化调节了Cps35与COMPASS复合体的结合.
- Cps35对于COMPASS的活体催化活性至关重要.
- 外源性Cps35可以恢复甲基化活性,使得缺乏H2B单基化的COMPASS.
- Cps35染色体协会取决于H2BK123单双化,但不是Set1.
- 对于H3K79三甲基化,也需要Cps35.
结论:
- Cps35起到关键的调解作用,将H2B单双化信号转化为H3甲基化.
- 这项研究揭示了H2B修饰和H3K4和H3K79甲基化中的COMPASS功能之间的分子联系.
- 这些发现提供了对复杂的交叉通话机制的洞察力,这些机制控制着基质子的修饰.
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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
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