温度触发了多的自我组装,使其成为多价值球形
Matthew R Dreher1, Andrew J Simnick, Karl Fischer
1Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
Journal of the American Chemical Society
|December 19, 2007
概括
热响应的弹性类多 (ELP) 自组合成体温以上的稳定菌根. 这些新的纳米粒子,具有配体,向癌细胞,提供热触发药物递送的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 纳米技术纳米技术
背景情况:
- 弹性类聚 (ELP) 是具有可调节性质的刺激响应生物聚合物.
- 块共聚合物的自组装成纳米结构对于药物输送和生物医学应用至关重要.
- 开发用于向治疗的热敏材料仍然是一个重大挑战.
研究的目的:
- 用N-终端联体对线性AB双块弹性素样多 (ELP) 进行基因合成和特征化.
- 为了研究这些ELP块共聚物的温度依赖的自我组装到球形小粒.
- 探索这些自我组装的细胞在向癌细胞传递方面的潜力.
主要方法:
- 使用递归定向绑定ELP块共聚物的遗传合成.
- 使用光散射,光光谱学和冷传输电子显微镜 (cryo-TEM) 进行自我组装的表征.
- 确定关键微粒温度,微粒大小和关键微粒度.
主要成果:
- 合成了十种ELP块共聚合物 (ELP(BC) 具有不同的分子量和块比.
- 在ELP (BC) 展示了两个阶段过渡:从unimer到micelle和从micelle到批量聚合物.
- 微粒的形成和大小可以通过聚合物长度和块比来控制,具有高稳定性 (4-8微米CMC).
- ELP(BC) 与配体形成了37-42°C范围内的多价值菌根,向癌细胞.
结论:
- 热敏的ELP块共聚合物可以自组装成体温以上的稳定球形小粒.
- 建立了控制微粒形成和属性的设计规则.
- 带有联体的ELP基细胞显示出热触发的有针对性的药物递送的前景,特别是在癌症治疗中.
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