通过希斯供需调节复制分叉的进展
Anja Groth1, Armelle Corpet, Adam J L Cook
1Laboratory of Nuclear Dynamics and Genome Plasticity, UMR218 CNRS/Institut Curie, 26 rue d'Ulm, 75248 Paris cedex 05, France.
Science (New York, N.Y.)
|December 22, 2007
概括
基因素陪伴素Asf1 (抗沉默功能1) 对于DNA复制至关重要. 它与MCM2-7螺旋酶协调基因组供应,确保适当的分叉进展和染色体稳定性.
科学领域:
- 分子生物学分子生物学
- 染色体生物学 染色体生物学
- 复制DNA复制DNA复制DNA复制
背景情况:
- 在真核生物中,DNA复制需要核细胞分裂和重组.
- 协调基因组动态与复制分叉进展对于染色体稳定至关重要.
研究的目的:
- 为了研究基因组辅导体Asf1在DNA复制中的作用.
- 为了阐明链接素供应和复制分叉进展的机制.
主要方法:
- 一个涉及Asf1,MCM2-7和H3-H4.4基因素的复合体的表征.
- 通过RNA干扰来消耗Asf1.1.
- 对DNA解缺陷的分析.
主要成果:
- 通过RNA干扰的Asf1枯竭会损害DNA在复制部位的解.
- 新素H3-H4的过度产生会损害Asf1功能和DNA解.
- 鉴定了一种Asf1-(H3-H4) -MCM2-7中间体,该中间体连接着基因素动态和复制.
结论:
- Asf1在协调基因素供应与DNA复制方面发挥着至关重要的作用.
- 复制分叉的进展是Asf1-(H3-H4) -MCM2-7复杂的微调复制分叉.
- 在复制分叉中,Asf1充当基因素受体和供体.
相关概念视频
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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
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02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
02:31Chromosome Replication
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01:29Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...