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相关概念视频

Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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相关实验视频

Updated: May 10, 2026

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
15:59

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc

Published on: May 1, 2010

预测Drosophila细分中的调节序列的表达模式.

Eran Segal1, Tali Raveh-Sadka, Mark Schroeder

  • 1Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel. eran@weizmann.ac.il

Nature
|January 4, 2008
PubMed
概括
此摘要是机器生成的。

一个新的热力学模型通过分析调节性DNA序列和转录因子相互作用,准确地预测元动物发育中的基因表达模式. 这种计算框架增强了对发育基因网络的理解.

更多相关视频

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
09:45

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Published on: June 3, 2011

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

相关实验视频

Last Updated: May 10, 2026

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
15:59

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc

Published on: May 1, 2010

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
09:45

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Published on: June 3, 2011

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

科学领域:

  • 发育生物学是发展生物学.
  • 系统生物学 系统生物学
  • 计算生物学是一种计算生物学.

背景情况:

  • 精确的时空基因表达对于元动物的发育至关重要.
  • 对转录控制网络的机制理解仍然不完整.

研究的目的:

  • 开发一种用于预测基因表达模式的新型热力学模型.
  • 阐明 cis 调节序列和转录因子 (TF) 如何协调发育.
  • 将模型应用于Drosophila melanogaster细分基因网络.

主要方法:

  • 开发了一种热力学模型,以计算基于 cis 调节序列和 TF 结合/表达的表达模式.
  • 应用该模型来分析Drosophila melanogaster中的cis调节模块 (CRM).
  • 研究了强弱TF结合点及其聚类的贡献.

主要成果:

  • 该模型准确地预测了CRM的表达模式.
  • 位置信息编码在调节序列和TF分布中.
  • 强和弱结合点都有助于高DNA占用率和突变强度.
  • 弱点的短距离同型集群使得合作结合,利表达模式.

结论:

  • 开发的热力学模型为理解基因调节提供了一个强大的工具.
  • 在开发过程中,定位信息被编码在监管DNA和TF格局中.
  • 通过集群站点进行合作绑定对于精确的发展模式至关重要.