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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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相关实验视频

Updated: Jul 8, 2026

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

通过随机光学重建显微镜进行三维超分辨率成像.

Bo Huang1, Wenqin Wang, Mark Bates

  • 1Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.

Science (New York, N.Y.)
|January 5, 2008
PubMed
概括

研究人员开发了用于纳米尺度成像的3D随机光学重建显微镜 (STORM). 该技术实现了细胞结构的高分辨率3D可视化,克服了轴向成像的先前局限性.

科学领域:

  • 生物物理学的生物物理.
  • 光学显微镜的使用方法
  • 纳米技术 纳米技术

背景情况:

  • 远场光显微镜在横向维度上实现了近分子分辨率 (20-30 nm).
  • 在三维 (3D) 成像中实现纳米级分辨率仍然是显微镜中的一个重大挑战.

研究的目的:

  • 展示一种用于高分辨率3D成像的3D随机光学重建显微镜 (STORM) 方法.
  • 为了克服纳米级显微镜的轴分辨率的局限性.

主要方法:

  • 利用光学形学来精确确定个体光体的轴向和侧向位置.
  • 采用可光切换探头的代,随机激活,用于高精度的3D定位.
  • 在不需要样本扫描的情况下构建3D图像.

主要成果:

  • 实现图像分辨率为20-30nm的横向尺寸和50-60nm的轴向尺寸.
  • 成功地解决了纳米细胞结构的3D形态.
  • 证明了纳米准确度在3D中定位单个光体.

结论:

  • 开发的3D STORM技术显著提升了纳米级成像能力.

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

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Super-resolution Imaging of the Bacterial Division Machinery

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

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Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

  • 这种方法可以在纳米尺度上对亚细胞结构进行详细的3D可视化.
  • 光学形结合随机激活为3D超分辨率显微镜提供了强大的方法.