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相关概念视频

Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
X-Inactivation01:58

X-Inactivation

The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.

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相关实验视频

Updated: Jul 8, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

对于DNA光开关行为来说,间隔不需要.

Daniel A Lutterman1, Abdellatif Chouai, Yao Liu

  • 1Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA.

Journal of the American Chemical Society
|January 9, 2008
PubMed
概括
此摘要是机器生成的。

这项研究揭示了一种双金属复合体作为DNA光开关而没有插入DNA. 这一发现挑战了光开关行为完全证实了DNA间隔的假设.

更多相关视频

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

相关实验视频

Last Updated: Jul 8, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

科学领域:

  • 协调化学 协调化学
  • 生物有机化学 生物有机化学
  • 摄影化学的使用.

背景情况:

  • 过渡金属复合体在DNA结合时表现出发光,通常被认为是合的.
  • 当与DNA结合时,DNA光开关复合体[Ru(bpy) 2(tpphz) ]2+ (1) 是发射性的.

研究的目的:

  • 为了研究非间接双金属复合物的DNA结合和发光性质[(bpy) 2Ru(tpphz) Ru(bpy) 2 4+ (2).
  • 为了确定光开关行为是否可以在没有DNA间隙的情况下发生.

主要方法:

  • 复合体2的光谱分析 (发射光谱) 在牛犊胸腺DNA (ct-DNA) 和鱼精子DNA的存在下.
  • 对粘度进行测量,以评估DNA间隙.
  • 密度函数理论 (DFT) 计算以了解电子属性.

主要成果:

  • 复合体2的发光强度增加了40倍,在与ct-DNA结合时呈红色转移.
  • 粘度测量和线程实验证实,复合体2不会插入DNA.
  • DFT计算支持观察到的发光特性,将它们与电子结构相关联.

结论:

  • 双金属复合体2通过非间接机制作为DNA光开关起作用.
  • 这项工作提供了第一个非间接金属复合物的例子,证明了光开关的行为.
  • 光开关的行为并不仅仅是DNA间隙的标志.