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Updated: Jul 8, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

在绿色光蛋白中用于激发状态质子转移的替代性质子受体:重新连接GFP

Deborah Stoner-Ma1, Andrew A Jaye, Kate L Ronayne

  • 1Department of Chemistry, Stony Brook University, Stony Brook, New York 11794-3400, USA.

Journal of the American Chemical Society
|January 9, 2008
PubMed
概括

绿色光蛋白 (GFP) 突变可以通过使激发状态质子转移 (ESPT) 恢复光. 涉及D148的低屏障键机制解释了双变异体的光恢复,简化了蛋白质质子转移要求.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...