PTRF-Cavin是一种保存的细胞质蛋白,对于洞穴的形成和功能是必不可少的.
Michelle M Hill1, Michele Bastiani, Robert Luetterforst
1Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia.
Cell
|January 15, 2008
概括
富含氨酸的超膜蛋白 (PTRF),也称为Cavin,对于形成细胞表面洞穴至关重要. 它的缺失阻止了洞穴的发展,并导致洞穴的退化.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 洞穴细胞是重要的等离子膜浸,参与诸如脂质调节和内细胞分裂等细胞过程.
- 控制洞穴形成和稳定的分子机制仍然不完全理解.
研究的目的:
- 为了确定对洞穴生物的生物发生和功能至关重要的蛋白质.
- 阐明Protopoly (proline-rich transmembrane protein) (PTRF),也称为Cavin,在洞穴形成中的作用.
主要方法:
- 使用比较蛋白质组学来识别潜在的洞穴层蛋白质.
- 研究了前列腺癌PC3细胞和斑马鱼笔弦发育中的PTRF-Cavin表达和局部化.
- 功能性测试涉及PTRF-Cavin敲击和表达研究,以评估洞穴形成和洞穴动力学.
主要成果:
- 确定了PTRF-Cavin作为一种关键蛋白质,在血膜上选择性地与成熟的洞穴相结合.
- 缺乏PTRF-Cavin表达与细胞和生物模型中缺少洞穴和平坦的血膜相关.
- PTRF-Cavin表达诱导了洞穴形成,而它的敲击降低了洞穴密度,增加了洞穴的移动性和降解.
结论:
- PTRF-Cavin对于洞穴的形成是不可或缺的.
- PTRF-Cavin在静止洞穴结构中的洞穴稳定中发挥着至关重要的作用.
- 这项研究确立了PTRF-Cavin作为洞穴生物发生和维护的必要组成部分.
相关概念视频
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K
Rab Proteins
4.0K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.0K
Vesicular Tubular Clusters
2.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.4K
Cotranslational Protein Translocation
8.3K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
8.3K
Clathrin Coated Vesicles
8.1K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.1K
Rab Cascades
2.8K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.8K


