德洛索菲拉Pgc蛋白抑制了原始生殖细胞中P-TEFb对染色素的招募

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Kazuko Hanyu-Nakamura¹, Hiroko Sonobe-Nojima, Akie Tanigawa

¹Laboratory for Germline Development, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.

|January 18, 2008

概括

一种叫做Pgc的蛋白质通过抑制转录来阻止生殖系祖先成为体细胞. 这种机制,涉及Pgcc.

科学领域:

发展生物学发展生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

胚胎细胞传递遗传信息,需要保护它们免于分化为体细胞.
抑制RNA聚合酶II (RNAPII) 依赖转录是保护生殖系祖先的关键机制.
在RNAPII中缺少CTD Ser 2酸化与胚胎细胞特异化期间压抑的转录有关.

研究的目的:

为了研究极性颗粒成分 (pgc) 蛋白在多索菲拉生殖细胞特征中的作用.
确定Pgc如何调节CTD Ser 2酸化和转录在生殖系祖先中的方法.
探索跨物种的生殖细胞特异化中P-TEFb抑制的保存机制.

主要方法:

在Drosophila胚胎中分析Pgc功能.
研究体细胞中异位Pgc表达的作用.
评估Pgc和P-TEFb之间的物理和遗传相互作用.
在新形成的极细胞中研究CTD Ser 2酸化.

主要成果:

Pgc蛋白对于抑制Drosophila生殖系祖先 (极细胞) 中的CTD Ser 2酸化至关重要.
在体细胞中异位Pgc表达可以抑制CTD Ser 2酸化.
Pgc在物理和遗传上与P-TEFb相互作用,抑制其对转录部位的招募.

结论:

Pgc作为P-TEFb的细胞类型特异性抑制剂,对Drosophila生殖细胞的特异性至关重要.
抑制P-TEFb可能是Drosophila和C. elegans.中生殖细胞特异性的保存机制.
这项研究揭示了Pgc在保护生殖系谱中的基本作用.

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