利斯特里奥林素O允许Listeria monocytogenes在巨细胞真空中复制
Cheryl L Birmingham1, Veronica Canadien, Natalia A Kaniuk
1Cell Biology Program, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.
Nature
|January 19, 2008
概括
在持续感染期间,Listeria monocytogenes可以在宿主细胞真空中复制. 这种新型的宽的Listeria含有菌体 (SLAP) 途径,依赖于Listeriolysin O,有助于细菌的生存.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 李斯特菌 (Listeria monocytogenes) 是一种细胞内病原体,通常在宿主细胞细胞质中复制.
- 在SCID小鼠的持续感染期间,L. monocytogenes被观察到真空体内,挑战了既定的感染范式.
研究的目的:
- 调查L. monocytogenes在持续感染期间空洞占用机制.
- 描述新型的宽的含有李斯特菌的菌体 (SLAP),以及它们在细菌复制和生存中的作用.
主要方法:
- 严重的综合免疫缺陷 (SCID) 小鼠感染L. monocytogenes.
- 在体外细胞培养实验中观察细菌复制和真空球的形成.
- 免疫光显微镜用于识别和表征LAMP1(+) 分区 (SLAP).
- 对细胞醇与SLAP中的细菌复制率的分析.
- 评估Listeriolysin O (LLO) 在SLAP形成和细菌存活中的作用.
主要成果:
- 发现L. monocytogenes在巨细胞中的非酸性,非降解性LAMP1(+) 分区内复制,称为SLAP.
- SLAP的形成取决于自.
- 与细胞质复制相比,SLAP中的细菌复制率降低.
- 形成毛孔的毒素Listeriolysin O (LLO) 对于SLAP的形成至关重要且足够.
- 一种LLO低的突变体显示了受损的细胞逃脱,但在SLAPs中复制速度缓慢.
结论:
- 在持续感染期间,L. monocytogenes可以在宿主巨细胞的真空中 (SLAP) 复制.
- LLO在促进L. monocytogenes真空复制和潜在地建立持久感染方面发挥着至关重要的作用.
- 这一发现揭示了L. monocytogenes的新生存策略,与其典型的细胞质复制不同.
相关概念视频
Lytic Cycle of Bacteriophages
60.2K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
60.2K
Lysogenic Cycle of Bacteriophages
49.8K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
49.8K
Defense Against Bacterial Pathogens
3.0K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
3.0K
Viral Replication: Lytic Cycle
3.0K
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
3.0K
Viral Replication: Lysogenic Cycle
2.7K
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
2.7K
Colonisation of Pathogens
77
Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...
77


