细胞循环控制的中心重复转录和异色色素组合的细胞循环控制
Ee Sin Chen1, Ke Zhang, Estelle Nicolas
1Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Nature
|January 25, 2008
概括
在裂变酵母中,RNA聚合酶II (RNAPII) 在S阶段中对中心重复的转录对于异色素蛋白组装至关重要. 这种调节的转录确保了适当的基因沉默和在细胞分裂中保持异色染色质.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 异色素调节染色体过程,包括转录沉默在真核生物.
- 在Schizosomyces pombe中,RNA聚合酶II (RNAPII) 中心重复的转录对于RNA干扰介导的异染色体组合至关重要.
研究的目的:
- 为了研究整个细胞循环中的色素动力学.
- 了解异色素对RNAPII转录在中心重复的影响.
主要方法:
- 细胞周期分析异色染色体动力学.
- 研究RNAPII转录在异性染色体形成中的作用.
- 分析基因组修饰 (H3K36甲基化,H3S10酸化) 和蛋白质复合体 (RITS,Clr4,Set2,凝固素) 在异色素蛋白调节中的功能.
主要成果:
- 存在一个特定的S阶段窗口,其中RNAPII优先转录中心重复的重复,由 heterochromatin强制执行.
- 在S阶段的RNAPII转录有助于RNA干扰和异染色质因子 (Ago1, Rik1) 的招募.
- 设置2-介导的H3K36甲基化与Clr4平行作用,用于异色染色体组合;H3S10酸化在线粒分裂过程中影响异色染色体,影响凝聚酶的招募和沉默.
结论:
- 至少有两种不同的机制将异色素向中心重复:RNAPII转录和染色体蛋白与H3K9甲基化结合.
- 这些机制协同确保通过细胞分裂维持异染色素,突出显示转录和表观遗传沉默之间的相互作用.
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