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Updated: Jul 7, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

通过多重RNAi查对人类乳腺细胞中的基本基因进行分析

Jose M Silva1, Krista Marran, Joel S Parker

  • 1Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Science (New York, N.Y.)
|February 2, 2008
PubMed
概括

研究人员选了成千上万个短发针RNAs (shRNAs),以发现人类乳腺癌细胞的漏洞. 这种方法确定了细胞存活的关键基因,并揭示了针对性治疗可以利用的特定弱点.

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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...