相关实验视频
Updated: Jul 7, 2026

08:44
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
Published on: January 22, 2019
没有轴的F1-ATPase在正确的方向上旋转
Shou Furuike1, Mohammad Delawar Hossain, Yasushi Maki
1Department of Physics, Faculty of Science and Engineering, Waseda University, Shinjuku-ku, Tokyo 169-8555, Japan.
概括
F1-腺三酸酶 (ATPase) 分子电机即使其中心轴显著缩短,也可以旋转. 这表明,固定的旋转轴或刚性轴对酶的扭矩产生和旋转运动并不必不可少.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- F1-腺三酸酶 (ATPase) 作为一种由ATP驱动的旋转分子电机.
- 它的结构包括一个中心的马子单元,它旋转在一个由交替的α和β子单元组成的定子筒中.
- 玛子单元的旋转轴穿透了定位器的中央腔.
研究的目的:
- 为了研究F1-ATPase旋转轴长度在其旋转功能中的作用.
- 为了确定电机旋转是否需要固定轴或刚性轴.
主要方法:
- 玛子单元的旋转轴的逐步切断.
- 对截断的F1-ATPase突变体的旋转运动的观察和分析.
- 评估扭矩生成和旋转速度.
主要成果:
- 所有截断的F1-ATPase突变体都显示出正确方向的旋转,表明扭矩产生.
- 较短的突变物表现出较低的平均旋转速度和偶尔的不规则运动.
- 酶在没有固定轴或刚性轴的情况下有效地旋转.
结论:
- 在不取消旋转运动的情况下,F1-ATPase旋转轴的长度可以显著减少.
- 即使旋转轴部分暴露时,扭矩产生和旋转也是可能的.
- 一个固定的枢纽或刚性轴不是F1-ATPase功能的先决条件.
更多相关视频
相关概念视频
ATP Synthase: Structure
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
ATP Synthase: Mechanism
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased ATP...
The Movement of Organelles and Vesicles
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
Mechanism of Ciliary Motion
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
Mechanism of Ciliary Motion
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
Forces Acting on Chromosomes
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...

