殖民地刺激因子1在细胞循环的G1阶段调节新型环林
H Matsushime1, M F Roussel, R A Ashmun
1Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
Cell
|May 17, 1991
概括
殖民地刺激因子1 (CSF-1) 在巨细胞G1阶段调节小鼠循环类 (CYL) 基因表达. CYL蛋白对于启动DNA合成至关重要,而CSF-1对于它们的稳定性和功能至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 巨细胞的增殖是由殖民地刺激因子1 (CSF-1) 调节的.
- 细胞周期进展涉及特定的基因表达和蛋白质调节.
- 环林类 (CYL) 蛋白质与细胞循环控制有关.
研究的目的:
- 为了研究小鼠环林类 (CYL) 基因在巨细胞循环调节中的作用.
- 确定CSF-1对CYL基因表达和蛋白质稳定性的影响.
- 为了阐明CYL蛋白在特定细胞周期阶段的功能.
主要方法:
- 分离了三只小鼠的CYL基因.
- 在G1阶段对CSF-1对CYL基因调节的分析.
- 对CYL蛋白降解和酸化的评估.
- 研究CYL蛋白与cdc2相关的多的关联.
主要成果:
- 三个孤立的CYL基因中有两个在G1期间由CSF-1调节.
- 在G1中缺少CSF-1会导致p36CYL蛋白质的快速降解.
- p36CYL降解与未能启动DNA合成有关.
- 进入S期的巨细胞不需要CSF-1或CYL基因表达来进行分裂.
- 在G1.1期间,p36CYL被酸化并与cdc2相关的多结合.
结论:
- 在G1阶段,CSF-1对于维持CYL蛋白的稳定性和功能至关重要.
- 在G1到S阶段过渡过程中,CYL基因起着至关重要的作用.
- 酸化和p36CYL与cdc2相关蛋白质的关联表明它在细胞循环调节中的作用.
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