一种裂变酵母B型环林,在细胞周期早期起作用
A Bueno1, H Richardson, S I Reed
1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell
|July 12, 1991
概括
一个新发现的裂变酵母基因,cig1+,编码了一个新的B型环林. 这种循环蛋白调节G1到S相过渡,与以前已知的细胞周期蛋白质不同.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 环素是细胞循环的关键调节剂.
- B型循环素通常参与了线粒细胞的进展.
- 在裂变酵母中cig1+的功能以前是未知的.
研究的目的:
- 为了克隆和表征裂变酵母基因cig1+.
- 为了确定cig1+蛋白在细胞循环中的作用.
- 为了研究cig1+与其他cyclin相比的功能区别.
主要方法:
- 基因克隆和cig1+基因的表征.
- 在裂变酵母中删除和过度表达的研究.
- 细胞周期进展和活力的分析.
主要成果:
- 裂变酵母基因cig1+编码了一个48kDa的蛋白质,与B型环素相似.
- 删除cig1+会导致G1到S阶段进展的显著延迟.
- 构成性过度表达cig1+是致命的,并阻止G1中的细胞.
- cig1+不会拯救缺乏CLN环林的S. cerevisiae.
结论:
- cig1+代表了一种新的B型循环素的类别.
- 这种循环蛋白在调节G1到S阶段过渡方面发挥着至关重要的作用.
- 在功能上,cig1+与已知的线粒体和G1环林不同.
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