相关实验视频
Updated: Jul 18, 2026
07:27
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
Published on: April 29, 2010
DNA复制发生在离散部位的伪核中,这些伪核由纯化DNA in vitro 组装在一起
1Cancer Research Campaign Molecular Embryology Group, Wellcome CRC Institute, Cambridge, England.
Cell
|July 26, 1991
概括
在Xenopus蛋提取物中,菌体兰巴达DNA的复制发生在离散的,集群的地点. 这种复制分叉的组织独立于真核细胞的DNA序列或核结构.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 众所周知,Xenopus蛋提取物中的菌体兰巴达DNA复制受细胞周期控制.
- 以前的研究表明,真核DNA复制是在核内组织的.
- 对于DNA序列和核结构在组织复制分叉中的作用尚不清楚.
研究的目的:
- 为了研究细菌羊DNA复制在Xenopus蛋提取物中的空间调节.
- 为了确定复制是否发生在离散站点,以及这些站点是否代表集群复制分叉.
- 评估复制分叉组织对真核DNA序列或核结构的依赖性.
主要方法:
- 在Xenopus蛋提取物中将菌体兰巴达DNA组装成伪核.
- 通过将生物-19-dUTP纳入新生DNA合成的可视化.
- 脉冲标记实验用于在S阶段跟踪复制焦点.
主要成果:
- 菌体的lambda DNA复制是空间调节的,发生在离散的位置.
- 这些离散站点对应于集群复制分叉.
- 观察到复制焦点在整个S阶段持续存在.
结论:
- 复制分叉的空间组织成离散的集群并不仅仅是真核细胞DNA.
- 这种组织不需要特定的真核细胞DNA序列.
- 复制分叉集群是独立于先前存在的遗传染色体或核结构.
相关概念视频
02:35Replication in Prokaryotes
Overview
02:35Replication in Prokaryotes
Overview
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The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
01:32Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...