结核细胞衍生细胞在结核纤维化和辅助途径的持久性发展
Denise P Kolditz1, Maurits C E F Wijffels, Nico A Blom
1Department of Cardiology, Leiden University Medical Center, Albinusdreef 2, PO Box 9600, 2300 RC, Leiden, The Netherlands.
Circulation
|March 12, 2008
概括
脊柱心衍生细胞 (EPDC) 对于形成结核纤维化至关重要,这通常会阻止辅助通路 (AP). 抑制EPDC导致AP和潜在的心律失常.
科学领域:
- 发育生物学是发展生物学.
- 心血管科学 心血管科学
- 电子生理学 电子生理学
背景情况:
- 绕过年纤维化的持续性心肌辅助通路 (APs) 背后的机制在很大程度上是未知的.
- 心衍生细胞 (EPDCs) 正在研究它们在结节纤维化和AP形成中的作用.
研究的目的:
- 调查EPDCs在结核纤维化形成中的作用.
- 确定EPDC抑制对AP和心脏电生理学发展的影响.
主要方法:
- 在卵胚胎中进行微手术,以机械抑制EPDC迁移.
- 在卵内和卵外心电图 (ECG) 和在汉堡 - 汉密尔顿阶段38-42记录的细胞外电图.
- 电生理学数据与心肌细胞 (MLC2a) 和纤维细胞 (periostin) 的分化标记物的相关性.
主要成果:
- 抑制EPDC显著缩短了PR间隔,并延长了ECG上的QRS持续时间.
- 100%的EPDC受阻心脏显示基第一心室激活,而野生类型的44%.
- 抑制EPDC导致大,周素阴性,MLC2a阳性APs与纤维化缺陷.
结论:
- EPDCs对于正确的环状纤维化形成至关重要.
- 抑制EPDC外生长导致显著的环纤维化缺陷和持久的APs.
- 这些胚胎AP可以作为产后复发性心律失常的基质.
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