昆虫的嗅觉受体是昆虫驱虫剂DEET的分子标
Mathias Ditzen1, Maurizio Pellegrino, Leslie B Vosshall
1Laboratory of Neurogenetics and Behavior, Rockefeller University, 1230 York Avenue, Box 63, New York, NY 10065 USA.
概括
抗昆虫剂N,N-二甲基多胺 (DEET) 通过抑制嗅觉感官神经元,阻止昆虫对气味的吸引力. 这种行动需要OR83b共同受体,识别新的驱逐剂开发的潜在目标.
科学领域:
- 神经科学是一个神经科学.
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
背景情况:
- N,N-二甲基多胺 (DEET) 是一种广泛使用的昆虫驱虫剂.
- 其精确的作用机制和分子点尚未完全理解.
研究的目的:
- 阐明DEET的分子点和作用机制.
- 研究DEET对昆虫嗅觉感官神经元和行为的影响.
主要方法:
- 在Anopheles gambiae和Drosophila melanogaster中嗅觉感官神经元的电生理记录.
- 行为测试测量Drosophila的气味吸引力.
- 涉及嗅觉共受体OR83b的基因分析.
主要成果:
- 发现DEET阻断了昆虫嗅觉神经元对有吸引力的气味的电生理反应.
- 在Drosophila中,DEET抑制了对食物气味的行为吸引力.
- 由于DEET对气味引起的电流的抑制作用,需要OR83b共受体.
- DEET抑制了由联结子单元和OR83b组成的气味受体复合体.
结论:
- 通过抑制依赖OR83b共同受体的特定异构体昆虫气味受体,DEET通过掩盖宿主气味来起作用.
- 识别这些分子点为设计改进的昆虫驱虫剂提供了基础.
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