来自热友古细菌的分子伴侣与真核蛋白t-复杂聚-1相关
J D Trent1, E Nimmesgern, J S Wall
1Howard Hughes Medical Institute, Yale School of Medicine, New Haven, Connecticut 06510.
Nature
|December 12, 1991
概括
来自古细菌的热冲击蛋白热友因子55 (TF55) 作为一个分子陪伴者. 它在进化上与真核蛋白t-复杂聚-1 (TCP1) 有关,这表明了一个新的护卫者类.
科学领域:
- 分子生物学分子生物学
- 进化生物学 进化生物学
- 生物化学 生物化学
背景情况:
- 有证据表明,古细菌组成部分和真核细胞质细胞质之间存在进化联系.
- 热冲击蛋白经常充当分子伴侣,帮助蛋白质折叠和稳定.
研究的目的:
- 研究热友古细菌Sulfolobus shibatae的主要热冲击蛋白 (TF55) 的功能和进化关系.
- 要确定TF55是否是一个分子伴侣,以及它是否与真核生物伴侣组件有关.
主要方法:
- 描述TF55的结构和寡合状态.
- 评估TF55在体外结合未折叠的多的能力.
- 分析了TF55的ATPase活性.
- 将TF55的主要结构与已知的伴侣蛋白和真核蛋白质进行比较.
主要成果:
- TF55是一种同类寡合体复合体,类似于沙佩罗宁环结构.
- TF55表现出分子伴侣活性,包括结合未折叠的多和具有ATPase活性.
- TF55的主要结构与已知的牧羊人蛋白无关,但与真核蛋白TCP1具有很高的同质性.
- 在Saccharomyces cerevisiae中TCP1是必不可少的,并且参与了线粒状的形成.
结论:
- 在热友古细菌中,TF55充当分子伴侣.
- TF55和真核细胞TCP1代表了一种新型的分子伴侣类,表明一种古老的进化关系.
- 这一发现扩大了我们对伴侣多样性和生命不同领域的演变的理解.
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