Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Metal-Semiconductor Junctions01:24

Metal-Semiconductor Junctions

The contact of metal and semiconductor can lead to the formation of a junction with either Schottky or Ohmic behavior.
Schottky Barriers
Schottky barriers arise when a metal with a work function (Φm) contacts a semiconductor with a different work function (Φs). Initially, electrons transfer until the Fermi levels of the metal and semiconductor align at equilibrium. For instance, if Φm > Φs, the semiconductor Fermi level is higher than the metal's before contact. The semiconductor's...
MOSFET: Enhancement Mode01:22

MOSFET: Enhancement Mode

Enhancement-mode MOSFETs are pivotal components in electronics, distinguished by their capacity to act as highly efficient switches. They are part of the larger family of metal-oxide Semiconductor Field-Effect Transistors (MOSFETs). They are available in two types: p-channel and n-channel, each tailored to specific polarity operations.
In their basic form, enhancement-mode MOSFETs are typically non-conductive when the gate-source voltage (Vgs) is zero. This default 'off' state means no current...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Sirt1 transgene delivery improves diabetes-impaired wound healing.

Bioactive materials·2026
Same author

Affinity Enhancement in Discrete Multivalent MegaMolecules.

Chembiochem : a European journal of chemical biology·2026
Same author

Engineering low-symmetry colloidal crystals with optical anisotropies.

Science advances·2026
Same author

Controlled Assembly of Vesicle-Based Superstructures Using Megamolecules.

ACS applied materials & interfaces·2026
Same author

Local protein detection with lateral flow assay read through tissue using X-ray excited luminescence chemical imaging.

Journal of biomedical optics·2025
Same author

33 Unresolved Questions in Nanoscience and Nanotechnology.

ACS nano·2025
Same journal

Switching Site Selectivity in Alkoxyamine Hydration: From Lone-Pair Direction to Solvent Network Dominance.

Journal of the American Chemical Society·2026
Same journal

A Topotactic Leap: 2D Layers to 3D Large-Pore Zeolite.

Journal of the American Chemical Society·2026
Same journal

Enhanced Hydrogen Evolution over Single-Atom Catalysts via Electrostatic Polarization in Contact-electro-catalysis.

Journal of the American Chemical Society·2026
Same journal

Tumor Acidity-Activatable Ionizable Lipid Nanoparticles for Selective Oncolytic Therapy.

Journal of the American Chemical Society·2026
Same journal

Alternating Magnetic Field Promotes Ammonia Cracking by Disrupting the Sabatier Limitation of Ruthenium Catalytic Species.

Journal of the American Chemical Society·2026
Same journal

Bulk Ferromagnetic Icosahedral Quasicrystals without Rapid Quenching.

Journal of the American Chemical Society·2026
查看所有相关文章

相关实验视频

Updated: Jul 6, 2026

Design, Fabrication, and Experimental Characterization of Plasmonic Photoconductive Terahertz Emitters
10:54

Design, Fabrication, and Experimental Characterization of Plasmonic Photoconductive Terahertz Emitters

Published on: July 8, 2013

一个调节的等离子开关.

W Paige Hall1, Jeffrey N Anker, Yao Lin

  • 1Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA.

Journal of the American Chemical Society
|April 12, 2008
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的使用calmodulin的等离子开关来检测蛋白质结构变化. 这种局部表面等离子体共振 (LSPR) 传感器显示可逆波长变化,使未标记蛋白质动态的实时分析成为可能.

更多相关视频

Evaluating Plasmonic Transport in Current-carrying Silver Nanowires
09:00

Evaluating Plasmonic Transport in Current-carrying Silver Nanowires

Published on: December 11, 2013

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

相关实验视频

Last Updated: Jul 6, 2026

Design, Fabrication, and Experimental Characterization of Plasmonic Photoconductive Terahertz Emitters
10:54

Design, Fabrication, and Experimental Characterization of Plasmonic Photoconductive Terahertz Emitters

Published on: July 8, 2013

Evaluating Plasmonic Transport in Current-carrying Silver Nanowires
09:00

Evaluating Plasmonic Transport in Current-carrying Silver Nanowires

Published on: December 11, 2013

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

科学领域:

  • 生物物理学的生物物理.
  • 纳米技术纳米技术
  • 频谱学是一种光谱学.

背景情况:

  • 卡尔莫杜林在结合时经历了显著的形状变化.
  • 局部表面等离子共振 (LSPR) 传感器对局部介电环境的变化敏感.
  • 开发实时,无标签的方法来监测蛋白质动态是至关重要的.

研究的目的:

  • 创建一个等离子开关来检测诱导的calmodulin形状变化.
  • 使用LSPR光谱技术进行无标签的蛋白质动态监测.
  • 开发一种高分辨率的LSPR光谱仪,用于灵敏地检测微小的波长变化.

主要方法:

  • 用一种新型的calmodulin构造 (cutinase-calmodulin-cutinase) 的LSPR传感器的功能化.
  • 使用高分辨率LSPR光谱仪 (1.5 x 10-2nm分辨率) 进行实时测量.
  • 监测可逆波长调制 (2-3纳米变化) 响应度变化.

主要成果:

  • 证明了LSPR灭绝最大 (lambdamax) 的可逆波长转移与度相关.
  • 与开放 (Ca2+结合) 相比,观察到卡尔莫杜林关闭 (无Ca2+) 的速度快4倍.
  • 在没有蛋白质标记的情况下使用LSPR实现了蛋白质结构动态的实时检测.

结论:

  • 开发的等离子开关能够有效地检测出calmodulin的可逆性,诱导的形状变化.
  • LSPR光谱学提供了一个强大的工具,用于对未标记的蛋白质动态进行无标签的实时分析.
  • 这项研究代表了LSPR光谱的首次应用,用于检测未标记蛋白质的可逆形状变化.