马管素是中心体的高度保存的组成部分
T Stearns1, L Evans, M Kirschner
1Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143.
Cell
|May 31, 1991
概括
高度保存的蛋白质 - - 马管氨酸对于细胞分裂和微管组织至关重要. 这种无处不在的蛋白质是中心体的关键组成部分,对细胞结构至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 管蛋白质形成微管,对于细胞结构和分裂至关重要.
- 马氨酸是氨酸的一个独特的亚家族,但其精确的作用和保护尚未完全理解.
研究的目的:
- 在各种物种中克隆和表征马管林基因.
- 为了研究马管素的保存,功能和局部化.
主要方法:
- 在各种生物体 (X. laevis,S. pombe,玉米,藻,酵母) 中进行基因克隆和表征.
- 蛋白质序列比较以评估保护性.
- 通过S. pombe的零突变对基因功能的分析.
- 免疫定位研究以确定细胞内的蛋白质定位.
主要成果:
- 玛布林基因在真核生物中高度保存,这表明它具有重要的,保存的功能.
- 在Schizosaccharomyces pombe gamma-tubulin中发生的无基因突变是致命的,这突显了它的重要性.
- 马管氨酸是中心体中较小的蛋白质组成部分,具体局限于周心状物质.
- 即使在微管脱聚合后,马管素仍然与中心体相关.
结论:
- 马管氨酸是一种无处不在且高度保存的蛋白质,对于微管细胞核形成至关重要.
- 它的定位在中心体上表明它在微管组织和细胞分裂中起着关键作用.
- 马管素是中心体微管核核化机制的组成部分.
相关概念视频
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Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...


