在酵母酵母中发生化特异性RNA剪接
J A Engebrecht1, K Voelkel-Meiman, G S Roeder
1Department of Biology, Yale University, New Haven, Connecticut 06511-8112.
Cell
|September 20, 1991
概括
这项研究揭示了酵母体中微变的转录后调节. MER1基因产物对于MER2基因剪接至关重要,这是半分裂的一个关键步骤.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 细胞调节 细胞调节 细胞调节
背景情况:
- 以前,人们认为酵母菌中介质调节主要是转录性的.
- MER2基因产物对于半变异是必不可少的.
研究的目的:
- 为了研究半体变异的转录后调节.
- 为了确定MER1基因在变中的作用.
主要方法:
- 在酵母中分析MER2基因转录和拼接.
- 对MER1和MER2基因相互作用的基因分析.
主要成果:
- MER2在线粒分裂和半分裂过程中都被转录,但只有在半分裂过程中才能有效地剪接.
- MER2的介质拼接取决于MER1基因产物,该基因产物只在介质细胞中产生.
- 在MER1蛋白中介于MER2的转录拼接.
结论:
- 酵母中的半转化是通过后转录来调节的.
- 而MER1蛋白对于MER2在变过程中进行剪接至关重要.
- MER1可能会调节其他介质基因的拼接.
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